通过逆转录PCR(RT-PCR)方法扩增HIV-1 pol基因的特定区域,并对PCR产物进行测序,将所测序列与数据库中的参考序列或共享序列比较,判断是否出现耐药相关的基因突变,并根据HIV-1耐药基因型解释系统的规则来评判对特定药物的耐药程度。
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This page (revision-2) was last changed on 21-Aug-2013 16:39 by 1614216108