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This page (revision-4) was last changed on 23-Aug-2013 11:36 by qinnan

This page was created on 21-Aug-2013 13:56 by qinnan

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At line 3 changed one line
双脱氧链终止测序法
　对菌（菌液、穿刺菌、平板菌）、质粒、PCR产物（原液、已纯化）等不同类型的样品进行测序。提供质粒提取、PCR纯化、测序引物设计、DNA序列的基本数据分析，以及长片段walking测序后的序列拼接等生物信息学分析服务。
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电泳有明显单一条带的PCR产物或质粒，及测序引物
（1） 菌液
样品需求量：500-1000 μL 新鲜菌液，也可提供 1-2 mL 菌液的沉淀菌体直接提取质粒；
（2） PCR 原液（未纯化）
a. 样品需求量：体积≥ 25 μL；
b. 样品浓度：≥ 20 ng/μL；
（3）质粒
a. 样品需求量：体积≥ 20 μL；
b. 样品浓度：≥100 ng/μL。
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秦楠 吴灵娇
倪淑君 吴灵娇
Version Date Modified Size Author Changes ... Change note
4 23-Aug-2013 11:36 0.585 kB qinnan to previous
3 22-Aug-2013 12:20 0.292 kB qinnan to previous | to last
2 21-Aug-2013 14:06 0.291 kB qinnan to previous | to last
1 21-Aug-2013 13:56 0.285 kB qinnan to last
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