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This page (revision-6) was last changed on 23-Aug-2013 11:39 by qinnan

This page was created on 22-Aug-2013 12:06 by qinnan

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通过片段化，加接头并扩增得到可以测序的文库
通过3'，5'加接头，片段化，逆转录，扩增并纯化得到可以测序的文库
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质检合格的RNA，且含量大于5微克
OD值260/280应在1.8～2.O之间，无肉眼可见污染；使用Agilent Bioanalyzer仪器检测，获得总RNA应28S:18S≥1，RIN≥7.5。 样品浓度：不低于100ng/uL。 样品总量：研究编码蛋白质：动物样品一次制备需10ug的总RNA，植物样品一次制备需15ug的总RNA；研究长链非编码RNA：无论动植物，请一次性提供30ug的高质量总RNA。样品请置于1.5mL管中，做好标记，使用封口膜封好，建议使用干冰运输，请勿反复冻融。
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DNA测序文库构建：2500元/样品
RNA测序文库构建：2500元/样品
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秦楠
倪淑君
Version Date Modified Size Author Changes ... Change note
6 23-Aug-2013 11:39 0.502 kB qinnan to previous
5 23-Aug-2013 11:28 0.494 kB qinnan to previous | to last
4 22-Aug-2013 12:19 0.282 kB qinnan to previous | to last
3 22-Aug-2013 12:09 0.281 kB qinnan to previous | to last
2 22-Aug-2013 12:08 0.277 kB qinnan to previous | to last
1 22-Aug-2013 12:06 0.277 kB qinnan to last
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