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This page (revision-5) was last changed on 23-Aug-2013 11:40 by qinnan

This page was created on 22-Aug-2013 12:07 by qinnan

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通过片段化，逆转录，加接头并扩增得到可以测序的文库
通过3'，5’加接头，逆转录，扩增，PAGE精确筛选区域片段并纯化得到可以测序的文库
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质检合格的microRNA，且含量大于5微克
OD值260/280应在1.8～2.O之间，无肉眼可见污染；使用Agilent Bioanalyzer仪器检测，获得总RNA应28S:18S≥1，RIN≥7.5。 样品浓度：不低于100ng/uL。 样品总量：请一次性提供至少10ug高质量总RNA。 样品请置于1.5mL管中，做好标记，使用封口膜封好，建议使用干冰运输，请勿反复冻融。
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DNA测序文库构建：3000元/样品
miRNA测序文库构建：3000元/样品
Version Date Modified Size Author Changes ... Change note
5 23-Aug-2013 11:40 0.46 kB qinnan to previous
4 23-Aug-2013 11:29 0.443 kB qinnan to previous | to last
3 22-Aug-2013 12:18 0.295 kB qinnan to previous | to last
2 22-Aug-2013 12:10 0.294 kB qinnan to previous | to last
1 22-Aug-2013 12:07 0.29 kB qinnan to last
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