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This page (revision-13) was last changed on 28-Dec-2012 17:21 by 1614216108

This page was created on 09-Apr-2012 11:32 by UnknownAuthor

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　　中文名称：M蛋白\\
　　中文名称：M蛋白测定\\
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(2) 血清蛋白醋酸纤维素膜电泳:M 蛋白在α2～γ 区形成浓密区带，用光密度描计己可出现基底较窄、高而尖锐的蛋白峰。在γ区，蛋白峰的高宽比值>2; 在β和α2 区> 1 。\\
(2)血清蛋白醋酸纤维素膜电泳:M 蛋白在α2～γ 区形成浓密区带，用光密度描计己可出现基底较窄、高而尖锐的蛋白峰。在γ区，蛋白峰的高宽比值>2; 在β和α2 区> 1 。\\
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(6) 免疫固定电泳:将待测血清或尿在载体上电泳，，使不同蛋白质形成电泳位置不同的区带。将特异性抗重链或抗轻链血清加于载体上，抗血清即可与相应的蛋白区带结合(例如抗kappa 链抗血请与kappa 轻链区带结合) ，形成抗原抗体复合物。洗去未与抗血清结合的区带，用氨基黑或丽春红染色，抗血清固定的区带即可呈色。\\
(7) 尿本-周蛋白( Bene• Jones protein) 即尿游离轻链检测:轻链-白蛋白-戊二醛免疫电泳法:取尿液5 ml ，加入2.0 g/L 牛血清白蛋白(BSA) 0.25ml ，再加0.5% 戊二醛0.25 ml 混匀，室温下30min。 尿液中的轻链能与BSA 在戊二醛的存在下结合。按常法与抗轻链血清进行对流免疫电泳，则检样与抗K 、λ 血清之间可产生白色沉淀线。此法阳性检出率为100% ，假阳性率为4% 。 尿中含有轻链200μg/ml 时即可出现阳性结果。\\
(6)免疫固定电泳:将待测血清在载体上电泳，使不同蛋白质形成电泳位置不同的区带。将特异性抗重链或抗轻链血清加于载体上，抗血清即可与相应的蛋白区带结合(例如抗kappa 链抗血请与kappa 轻链区带结合) ，形成抗原抗体复合物。洗去未与抗血清结合的区带，用氨基黑或丽春红染色，抗血清固定的区带即可呈色。\\
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[血.免疫球蛋白]\\[临床免疫学检验]
[血.免疫球蛋白]
Version Date Modified Size Author Changes ... Change note
13 28-Dec-2012 17:21 1.277 kB 1614216108 to previous
12 05-Dec-2012 14:40 1.31 kB 1614216108 to previous | to last 血.免疫功能测定 ==> 血.免疫功能检验
11 05-Dec-2012 14:40 1.31 kB 1614216108 to previous | to last 血.免疫球蛋白 ==> 血.免疫功能测定
10 04-Dec-2012 14:40 1.309 kB 1614216108 to previous | to last
9 16-Jul-2012 15:25 1.583 kB 1614216108 to previous | to last 免疫球蛋白 ==> 血.免疫球蛋白
8 16-Jul-2012 14:27 1.581 kB 1614216108 to previous | to last 血液.M蛋白测定 ==> M蛋白测定
7 09-May-2012 14:13 1.581 kB 1614216108 to previous | to last
6 09-May-2012 14:13 0.028 kB 1614216108 to previous | to last
5 09-May-2012 14:11 1.581 kB 1614216108 to previous | to last M蛋白测定 ==> 血液.M蛋白测定
4 09-May-2012 13:59 1.581 kB 1614216108 to previous | to last
3 25-Apr-2012 11:33 1.561 kB 1614216108 to previous | to last
2 12-Apr-2012 13:47 1.58 kB 1614216108 to previous | to last
1 09-Apr-2012 11:32 1.561 kB UnknownAuthor to last
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