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This page (revision-7) was last changed on 23-Aug-2013 11:26 by qinnan

This page was created on 21-Aug-2013 11:02 by qinnan

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合格的DNA,RNA，且含量大于5微克
DNA：基因组完整、无降解，电泳结果基因组DNA主带应在入-Hind III digest最大条带23kb左右，且主带清晰，无弥散；OD值260/280应在1.8～2.0之间：无蛋白质、RNA或肉眼可见杂质污染。 RNA：OD值260/280应在1.8～2.O之间，无肉眼可见污染；使用Agilent Bioanalyzer仪器检测，获得总RNA应28S:18S≥1，RIN≥7.5。 样品浓度：不低于100ng/uL。 DNA样品总量：每次样品制备需要大于3ug的样品。为保证实验质量及延续性，请一次性提供至少6ug的样品。如需多次制备样品，按照制备次数计算样品总量。 RNA样品总量：研究编码蛋白质：动物样品一次制备需10ug的总RNA，植物样品一次制备需15ug的总RNA；研究长链非编码RNA：无论动植物，请一次性提供30ug的高质量总RNA；研究小RNA：请一次性提供至少10ug高质量总RNA。 样品请置于1.5mL管中，做好标记，使用封口膜封好，DNA建议使用干冰或冰袋运输，RNA建议使用干冰运输，请勿反复冻融。
Version Date Modified Size Author Changes ... Change note
7 23-Aug-2013 11:26 1.079 kB qinnan to previous
6 23-Aug-2013 11:26 0.634 kB qinnan to previous | to last
5 22-Aug-2013 12:19 0.414 kB qinnan to previous | to last
4 21-Aug-2013 11:11 0.409 kB qinnan to previous | to last
3 21-Aug-2013 11:09 0.397 kB qinnan to previous | to last
2 21-Aug-2013 11:06 0.345 kB qinnan to previous | to last
1 21-Aug-2013 11:02 0.017 kB qinnan to last
« This page (revision-7) was last changed on 23-Aug-2013 11:26 by qinnan