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This page (revision-7) was last changed on 23-Aug-2013 11:26 by qinnan

This page was created on 21-Aug-2013 11:02 by qinnan

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基于半导体芯片的新一代革命性测序技术
Ion Torrent平台是基于半导体技术的台式高通量测序仪。该平台使用了一种布满小孔的高密度半导体芯片，一个小孔就是一个测序反应池，孔底部带有感应器。当DNA聚合酶把核苷酸聚合到延伸的DNA链上时，会释放出一个氢离子，反应池中的PH发生改变，位于池下的离子感受器就会感受到信号，把化学信号直接转化为数字信号，从而读出DNA序列。与其它新一代测序仪相比，Ion Torrent平台不需要激发光、CCD成像仪及荧光标记，能直接并快速“读”出DNA序列，具有简单、快速、准确、灵活和低成本等显著优势，是中等规模测序项目的最佳选择。
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DNA测序、RNA测序、宏基因组测序、甲基化测序、外显子捕获测序、ChIP-Seq等，能够为基因调控分析、转录组分析、SNP位点、结构变异分析和疾病分析等提供迅捷、庞大、准确的信息数据。
1、小基因组测序（如：微生物和病毒的从头测序和重测序；线粒体测序等）
2、扩增子重测序（如：16S宏基因组测序）
3、靶向重测序
Version Date Modified Size Author Changes ... Change note
7 23-Aug-2013 11:26 1.079 kB qinnan to previous
6 23-Aug-2013 11:26 0.634 kB qinnan to previous | to last
5 22-Aug-2013 12:19 0.414 kB qinnan to previous | to last
4 21-Aug-2013 11:11 0.409 kB qinnan to previous | to last
3 21-Aug-2013 11:09 0.397 kB qinnan to previous | to last
2 21-Aug-2013 11:06 0.345 kB qinnan to previous | to last
1 21-Aug-2013 11:02 0.017 kB qinnan to last
« This page (revision-7) was last changed on 23-Aug-2013 11:26 by qinnan