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This page (revision-5) was last changed on 19-Aug-2013 17:53 by 吴珊珊

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本试剂盒应用双抗体夹心法测定标本中人白介素6(IL-6)水平。用纯化的人白介素6(IL-6)抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入白介素6(IL-6),再与HRP标记的白介素6(IL-6)抗体结合,形成抗体-抗原-酶标抗体复合物,经过彻底洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的白介素6(IL-6)呈正相关。用酶标仪在450nm波长下测定吸光度(OD值),通过标准曲线计算样品中人白介素6(IL-6)浓度。
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酶标仪
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(也可以选择正常值范围或国际标准)
6.25-200pg/ml
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白介素6(IL-6)是一类多功能的蛋白,在宿主防御,急性期反应,免疫反应,造血功能,神经系统等方面起到重要的作用。白介素6根据来源不同是分子量从21到28kDa不同的单链蛋白。白介素6在多种细胞中都有表达,如T细胞、巨噬细胞、纤维原细胞、肝细胞、血管内皮细胞等,IL-6在B细胞向Ig分泌细胞的转化过程中起到重要的作用,参与了淋巴细胞和单核细胞的分化,诱导神经细胞在B细胞,T细胞,肝细胞,造血干细胞以及中枢神经系统的分化作用。此外,肌肉运动收缩作用后IL-6会被释放到血液中,进而能促进脂肪的分解并改善机体的胰岛素耐受性。检测不同人群体内IL-6水平具有很大的研究意义
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人血清样本,避免反复冻融或溶血,血清量≧200ul
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200元/人/指标
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吴珊珊
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[XXXXX平台]
[细胞生物学平台]
Version Date Modified Size Author Changes ... Change note
5 19-Aug-2013 17:53 0.755 kB 吴珊珊 to previous
4 19-Aug-2013 17:52 0.771 kB 吴珊珊 to previous | to last
3 19-Aug-2013 17:29 0.439 kB 吴珊珊 to previous | to last
2 19-Aug-2013 17:28 0.439 kB 吴珊珊 to previous | to last
1 19-Aug-2013 17:24 0.186 kB 吴珊珊 to last
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