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This page (revision-5) was last changed on 19-Aug-2013 17:53 by 吴珊珊

This page was created on 19-Aug-2013 17:24 by 吴珊珊

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本试剂盒应用双抗体夹心法测定标本中人白介素6(IL-6)水平。用纯化的人白介素6(IL-6)抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入白介素6(IL-6),再与HRP标记的白介素6(IL-6)抗体结合,形成抗体-抗原-酶标抗体复合物,经过彻底洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的白介素6(IL-6)呈正相关。用酶标仪在450nm波长下测定吸光度(OD值),通过标准曲线计算样品中人白介素6(IL-6)浓度。
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酶标仪
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Version Date Modified Size Author Changes ... Change note
5 19-Aug-2013 17:53 0.755 kB 吴珊珊 to previous
4 19-Aug-2013 17:52 0.771 kB 吴珊珊 to previous | to last
3 19-Aug-2013 17:29 0.439 kB 吴珊珊 to previous | to last
2 19-Aug-2013 17:28 0.439 kB 吴珊珊 to previous | to last
1 19-Aug-2013 17:24 0.186 kB 吴珊珊 to last
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