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This page (revision-7) was last changed on 03-Dec-2012 11:54 by 1614216108

This page was created on 09-Apr-2012 16:59 by UnknownAuthor

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  英文名称:sickle cell solubility test\\
  英文名称:[sickle cell solubility test]\\
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   镰状细胞贫血是出现异常HbS的常染色体显性遗传疾病,当血氧过低时HbS相互聚集形成多聚体,当足够的多聚体形成时,红细胞即由双面凹盘状变成镰刀型,镰变的红细胞僵硬变形性差,容易被破坏,并使血液粘滞性增加。镰状细胞溶解度试验是利用血红蛋白液在连二亚硫酸钠磷酸盐溶液中,HbS经连二亚硫酸钠还原去氧后在磷酸盐溶液中溶解度下降,HbS出现沉淀,通过检测去沉淀后血红蛋白的量的变化得到HbS的含量百分率。\\
  镰状细胞贫血是出现异常HbS的常染色体显性遗传疾病,当血氧过低时HbS相互聚集形成多聚体,当足够的多聚体形成时,红细胞即由双面凹盘状变成镰刀型,镰变的红细胞僵硬变形性差,容易被破坏,并使血液粘滞性增加。镰状细胞溶解度试验是利用血红蛋白液在连二亚硫酸钠磷酸盐溶液中,HbS经连二亚硫酸钠还原去氧后在磷酸盐溶液中溶解度下降,HbS出现沉淀,通过检测去沉淀后血红蛋白的量的变化得到HbS的含量百分率。\\
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健康人血红蛋白溶解度为88%-100%。
  健康人血红蛋白溶解度为88%-100%。
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无。
  无。
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无。
  无。
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本试验用于诊断HbS病,并可与HbS、HbD、 HbO等血红蛋白进行鉴别。近期大量输血、试剂过期可出现假阴性。血红蛋白液加入试剂后应充分混合和反应时间要够。去氧血红蛋白在波长425nm-430nm和555nm各有一吸收峰,而425nm-430nm吸收峰大,故选428nm作为测定波长。\\
  本试验用于诊断HbS病,并可与HbS、HbD、 HbO等血红蛋白进行鉴别。近期大量输血、试剂过期可出现假阴性。血红蛋白液加入试剂后应充分混合和反应时间要够。去氧血红蛋白在波长425nm-430nm和555nm各有一吸收峰,而425nm-430nm吸收峰大,故选428nm作为测定波长。\\
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无特殊要求。\\
  无特殊要求。\\
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肝素抗凝全血标本。\\
  肝素抗凝全血标本。\\
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[贫血及溶血检验]
Version Date Modified Size Author Changes ... Change note
7 03-Dec-2012 11:54 0.802 kB 1614216108 to previous
6 03-Dec-2012 11:54 0.8 kB 1614216108 to previous | to last
5 12-Jul-2012 17:05 0.796 kB 1614216108 to previous | to last
4 03-May-2012 16:06 0.807 kB 1614216108 to previous | to last
3 25-Apr-2012 11:12 0.785 kB 1614216108 to previous | to last
2 12-Apr-2012 14:21 0.786 kB 1614216108 to previous | to last
1 09-Apr-2012 16:59 0.763 kB UnknownAuthor to last
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