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This page (revision-7) was last changed on 14-Oct-2013 14:05 by 1614216108

This page was created on 09-Apr-2012 17:11 by UnknownAuthor

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中文名称:酸化甘油溶血试验\\
英文名称:acidified glycerin hemolysis test,AGLT\\
  中文名称:酸化甘油溶血试验\\
  英文名称:acidified glycerin hemolysis test,AGLT\\
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酸化甘油溶血试验当甘油存在于低渗溶液氯化钠磷酸缓冲液时,可阻止低渗溶液中的水快速进入红细胞内,减慢溶血过程。但甘油与膜脂质又有亲和性,可使膜脂质减少。当红细胞膜蛋白和膜脂质有缺陷时,它们在pH6.85的甘油缓冲液中比正常红细胞溶解的快,导致红细胞悬液的吸光度降至50%的时间(ALGT50)明显缩短。正常红细胞加入酸化甘油30min后光密度仅有轻度下降,而遗传性球性细胞增多症患者红细胞很快降到一半以下,ALGT50多为40~100s。这种差异可能与红细胞膜、脂质成分、流动以及胞膜电荷有关。\\
  酸化甘油溶血试验当甘油存在于低渗溶液氯化钠磷酸缓冲液时,可阻止低渗溶液中的水快速进入红细胞内,减慢溶血过程。但甘油与膜脂质又有亲和性,可使膜脂质减少。当红细胞膜蛋白和膜脂质有缺陷时,它们在pH6.85的甘油缓冲液中比正常红细胞溶解的快,导致红细胞悬液的吸光度降至50%的时间(ALGT50)明显缩短。正常红细胞加入酸化甘油30min后光密度仅有轻度下降,而遗传性球性细胞增多症患者红细胞很快降到一半以下,ALGT50多为40~100s。这种差异可能与红细胞膜、脂质成分、流动以及胞膜电荷有关。\\
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本试验对诊断遗传性球性细胞增多症是比较特异和敏感的实验室检查,也是进行家系调查的较理想的方法。\\
  本试验对诊断遗传性球性细胞增多症是比较特异和敏感的实验室检查,也是进行家系调查的较理想的方法。\\
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应注意试剂的量和温度的准确,以保证结果的可靠性。同时做正常对照。\\
  应注意试剂的量和温度的准确,以保证结果的可靠性。同时做正常对照。\\
Version Date Modified Size Author Changes ... Change note
7 14-Oct-2013 14:05 0.702 kB 1614216108 to previous
6 12-Jul-2012 16:37 0.749 kB 1614216108 to previous | to last
5 12-Jul-2012 16:37 0.74 kB 1614216108 to previous | to last
4 03-May-2012 16:13 0.76 kB 1614216108 to previous | to last
3 25-Apr-2012 11:07 0.728 kB 1614216108 to previous | to last
2 12-Apr-2012 14:29 0.729 kB 1614216108 to previous | to last
1 09-Apr-2012 17:11 0.719 kB UnknownAuthor to last
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