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This page (revision-7) was last changed on 14-Jan-2013 16:17 by 1614216108

This page was created on 13-Apr-2012 14:25 by UnknownAuthor

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%%tab-选择素selectin
%%tab-选择素测定
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　　P-选择素8 -38μg/L ， E-选择素17 -31μg/L ，L选择素参考值暂缺。\\
　　P-选择素8-38μg/L ， E-选择素17-31μg/L ，L选择素参考值暂缺。\\
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%%tab-医学决定水平
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　　采用双抗体夹心ELISA 法。根据检测目的将抗人选择素(P- 、L或E-选择素)单克隆抗体包被于聚苯乙烯反应板微孔，待测样本中的选择素(P- 、L或E-选择素)与之结合，加入生物素化的抗选择素抗体，此抗体与结合在单抗上的选择素结合形成免疫复合物，未结合的成分被洗去。再加辣根过氧化物酶( HRP) 标记的链霉亲合素与生物素化的抗选择素抗体结合，洗去未结合的标记物质，加酶底物/色原显色。检样中选择素的浓度在一定范围内与吸光度(A) 值成正比。\\
　　采用双抗体夹心ELISA 法。根据检测目的将抗人选择素(P-、L或E-选择素)单克隆抗体包被于聚苯乙烯反应板微孔，待测样本中的选择素(P-、L或E-选择素)与之结合，加入生物素化的抗选择素抗体，此抗体与结合在单抗上的选择素结合形成免疫复合物，未结合的成分被洗去。再加辣根过氧化物酶(HRP) 标记的链霉亲合素与生物素化的抗选择素抗体结合，洗去未结合的标记物质，加酶底物/色原显色。检样中选择素的浓度在一定范围内与吸光度(A) 值成正比。\\
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%%tab-病人准备
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%%tab-标本采集和运送要求
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Version Date Modified Size Author Changes ... Change note
7 14-Jan-2013 16:17 1.004 kB 1614216108 to previous
6 04-Dec-2012 15:10 1.123 kB 1614216108 to previous | to last 选择素selectin ==> 选择素测定
5 04-Dec-2012 15:10 1.123 kB 1614216108 to previous | to last
4 04-May-2012 16:06 1.134 kB 1614216108 to previous | to last 选择素 selectin ==> 选择素selectin
3 04-May-2012 16:02 1.134 kB 1614216108 to previous | to last
2 23-Apr-2012 16:13 1.111 kB 1614216108 to previous | to last
1 13-Apr-2012 14:25 1.121 kB UnknownAuthor to last
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