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This page (revision-9) was last changed on 05-Dec-2012 15:07 by 1614216108

This page was created on 10-Apr-2012 14:54 by UnknownAuthor

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  人群变异:25.5%\\
  人群变异:25.5%
/% 
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腺苷脱氨酶是一种与机体细胞免疫活性有重要关系的核酸代谢酶。\\
__肝脏疾病:__\\
__肝脏疾病__\\
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__血液病\\__
__血液病__\\
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鉴别结核性和非结核性胸腹腔积液是临床病因诊断的一个常见难题,1978年Piras等报告结核性胸水中ADA活性明显高于其他原因所致的胸水,提出胸水ADA活性可用于结核性和癌性胸膜炎的鉴别诊断。费晓峰等对72例不同胸水ADA活性测定,以40U/L为界,结核性均超过此值,恶性者97.9%低于此值,小于35U/L者占93.2%,故作者认为胸水ADA大于40U/L提示结核性,小于35U/L提示恶性或非结核性。\\
  鉴别结核性和非结核性胸腹腔积液是临床病因诊断的一个常见难题,1978年Piras等报告结核性胸水中ADA活性明显高于其他原因所致的胸水,提出胸水ADA活性可用于结核性和癌性胸膜炎的鉴别诊断。费晓峰等对72例不同胸水ADA活性测定,以40U/L为界,结核性均超过此值,恶性者97.9%低于此值,小于35U/L者占93.2%,故作者认为胸水ADA大于40U/L提示结核性,小于35U/L提示恶性或非结核性。
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(3)比色法剸:5~25U/ml\\
(3)比色法剸:5~25U/ml
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%%tab-医学决定水平
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%%tab-危急值范围
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  最新对第四代测试方法的改进为偶联PNP,XOD和过氧化物酶(Peroxidase, POD)反应。ADA酶解腺苷(Adenosine)脱氨产生次黄苷(Inosine);再通过PNP的作用,生成次黄嘌呤(Hypoxanthine);后者在XOD氧化下生成尿酸(Uric Acid)和过氧化氢(H2O2);最后在POD的作用下H2O2再与N-Ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline (EHSPT)、4-氨基安替比林(4-aminoantipyrine, 4-AA)反应(Trinder氏反应),生成紫红色的有色醌(Quinone dye)。通过动态测量有色醌550nm处吸光度上升的速度来测算ADA的活性。从反应原理可知NH4+对该法无影响。反应具有测定精密度高,抗干扰能力强,适合自动化快速测定的特点,为临床常规开展ADA检测应用创造了有利条件。\\
  最新对第四代测试方法的改进为偶联PNP,XOD和过氧化物酶(Peroxidase, POD)反应。ADA酶解腺苷(Adenosine)脱氨产生次黄苷(Inosine);再通过PNP的作用,生成次黄嘌呤(Hypoxanthine);后者在XOD氧化下生成尿酸(Uric Acid)和过氧化氢(H2O2);最后在POD的作用下H2O2再与N-Ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline (EHSPT)、4-氨基安替比林(4-aminoantipyrine, 4-AA)反应(Trinder氏反应),生成紫红色的有色醌(Quinone dye)。通过动态测量有色醌550nm处吸光度上升的速度来测算ADA的活性。从反应原理可知NH4+对该法无影响。反应具有测定精密度高,抗干扰能力强,适合自动化快速测定的特点,为临床常规开展ADA检测应用创造了有利条件。
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抽血前的一周内应该保持正常的生活状态,以使身体处于一个稳定的代谢水平;采血前应使受检者保持平静、松弛、避免剧烈活动,空腹十小时以上。\\
  抽血前的一周内应该保持正常的生活状态,以使身体处于一个稳定的代谢水平;采血前应使受检者保持平静、松弛、避免剧烈活动,空腹十小时以上。
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采集后注入试管中,防止剧烈震荡,并尽快送往化验室。\\
采集后注入试管中,防止剧烈震荡,并尽快送往化验室。
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[血清酶]\\[肝功能]
[血清酶]
Version Date Modified Size Author Changes ... Change note
9 05-Dec-2012 15:07 3.849 kB 1614216108 to previous 血清酶 ==> 血.酶及相关物质检验
8 05-Dec-2012 15:07 3.842 kB 1614216108 to previous | to last
7 24-Aug-2012 14:54 3.955 kB 1614216108 to previous | to last
6 18-Jul-2012 16:54 3.952 kB 1614216108 to previous | to last 酶 ==> 血清酶
5 14-May-2012 10:28 3.95 kB 1614216108 to previous | to last
4 07-May-2012 14:27 3.957 kB 1614216108 to previous | to last
3 23-Apr-2012 13:51 3.936 kB 1614216108 to previous | to last
2 12-Apr-2012 15:23 3.93 kB 1614216108 to previous | to last
1 10-Apr-2012 14:54 3.896 kB UnknownAuthor to last
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