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This page (revision-6) was last changed on 18-Jan-2013 15:14 by 1614216108

This page was created on 11-May-2012 14:21 by 1614216108

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(1)滴定法:用0.05mol/L氢氧化钠滴定空白管和测定管,至呈持久的浅灰蓝色为止,记录滴定用去的氢氧化钠毫升数。
(2)比浊法:\\ ①于试管内加入4ml橄榄油乳剂,37℃预温5min,加入0.05ml血清,立即颠倒混合5次(切勿用力振摇),迅速用分光光度计比浊,以 Tris缓冲液调零点,在400nm波长读取吸光度为A。\\②将此管置于 37℃水浴保温10min,然后同上读取吸光度为A。\\③如果酶活力很高,在保温期间,底物全部变清,则应将待测血清用Tris缓冲液作适当稀释后重新操作。\\④标准曲线制作,取试管4只,分别加入橄榄油乳剂1.0、2.0、3.0及
4.0ml,然后用 Tris缓冲液补充到4.0ml,相当于甘油三酯浓度分别为0.17、0.34、0.51及 0.68μmol,同上比浊,绘制标准曲线。
(1)滴定法:用0.05mol/L氢氧化钠滴定空白管和测定管,至呈持久的浅灰蓝色为止,记录滴定用去的氢氧化钠毫升数。\\
(2)比浊法:\\ ①于试管内加入4ml橄榄油乳剂,37℃预温5min,加入0.05ml血清,立即颠倒混合5次(切勿用力振摇),迅速用分光光度计比浊,以 Tris缓冲液调零点,在400nm波长读取吸光度为A。\\②将此管置于 37℃水浴保温10min,然后同上读取吸光度为A。\\③如果酶活力很高,在保温期间,底物全部变清,则应将待测血清用Tris缓冲液作适当稀释后重新操作。\\④标准曲线制作,取试管4只,分别加入橄榄油乳剂1.0、2.0、3.0及4.0ml,然后用 Tris缓冲液补充到4.0ml,相当于甘油三酯浓度分别为0.17、0.34、0.51及 0.68μmol,同上比浊,绘制标准曲线。 \\
Version Date Modified Size Author Changes ... Change note
6 18-Jan-2013 15:14 1.145 kB 1614216108 to previous
5 05-Dec-2012 15:07 1.201 kB 1614216108 to previous | to last 血清酶 ==> 血.酶及相关物质检验
4 05-Dec-2012 15:07 1.194 kB 1614216108 to previous | to last 脂肪酶测定 ==> 血清脂肪酶测定
3 17-Jul-2012 16:48 1.194 kB 1614216108 to previous | to last 酶 ==> 血清酶
2 14-May-2012 10:28 1.192 kB 1614216108 to previous | to last
1 11-May-2012 14:21 1.19 kB 1614216108 to last
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