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血液疟原虫.png 95.8 kB 1 01-Apr-2012 14:12 1614216108

This page (revision-8) was last changed on 24-Dec-2012 10:56 by 1614216108

This page was created on 09-Apr-2012 09:42 by 1614216108

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__项目介绍 :__\\
疟原虫是导致人类疟疾的病原体,由雌性按蚊传播。寄生于人体的疟原虫共有4种,即恶性疟原虫、间日疟原虫、三日疟原虫和卵形疟原虫。在我国,引起疟疾发病的主要是间日疟和恶性疟。从患者周围血液中检出疟原虫是疟疾确诊的依据。选择合适的采血时间:恶心疟在发作开始时,间日疟在发作后数小时至10余小时采血。\\
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%%tab-影响因素
%%tab-方法学评价
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!一、项目介绍\\
疟原虫是导致人类疟疾的病原体,由雌性按蚊传播。寄生于人体的疟原虫共有4种,即恶性疟原虫、间日疟原虫、三日疟原虫和卵形疟原虫。在我国,引起疟疾发病的主要是间日疟和恶性疟。从患者周围血液中检出疟原虫是疟疾确诊的依据。选择合适的采血时间:恶心疟在发作开始时,间日疟在发作后数小时至10余小时采血。\\
!二、方法学评价\\
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|病原学诊断| | |
|1.血膜染色镜检|简便易行,结果可靠,成本低,至今是最常用的方法,\\薄血膜易识别和鉴别虫种。但原虫血症低于50-500个原虫/μl血,易产生误诊或漏诊。\\另外,选择适宜的采血时间对提高检出率非常重要。
|2.血沉棕黄色定量分析法|此法通过浓缩作用,其敏感性比普通镜检法高8倍以上,\\但看不到疟色素,易将染料渣滓误以为疟原虫。另外费用较高,对实验器材有特殊要求。
|__病原学诊断__|
|1.血膜染色镜检|简便易行,结果可靠,成本低,至今是最常用的方法,薄血膜易识别和鉴别虫种。\\但原虫血症低于50-500个原虫/μl血,易产生误诊或漏诊。另外,选择适宜\\的采血时间对提高检出率非常重要。
|2.血沉棕黄色定量分析法|此法通过浓缩作用,其敏感性比普通镜检法高8倍以上,但看不到疟色素,\\易将染料渣滓误以为疟原虫。另外费用较高,对实验器材有特殊要求。
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|免疫学诊断| | |
|__免疫学诊断__|
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|分子生物学技术| | |
|__分子生物学技术__|
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|2.PCR|敏感性和特异性很高,能确诊现症病人,有助于诊断混合感染,可区分交叉反应,是有广泛应用前景的检测手段。
|3.聚合酶链反应-酶联\\免疫吸附试验(PCR-ELISA)|特异性高,敏感性强,检测极限达到1.5个/μl血。缺点是实验成本高,限制了临床广泛应用
|2.PCR|敏感性和特异性很高,能确诊现症病人,有助于诊断混合感染,可区分交叉反应,\\是有广泛应用前景的检测手段。
|3.聚合酶链反应-酶联\\免疫吸附试验(PCR-ELISA)|特异性高,敏感性强,检测极限达到1.5个/μl血。缺点是实验成本高,限制了临床\\广泛应用
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!!病人准备:\\
/%
%%tab-病人准备
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!!标本采集和运送要求:\\
/%
%%tab-标本采集和运送要求
Version Date Modified Size Author Changes ... Change note
8 24-Dec-2012 10:56 1.397 kB 1614216108 to previous
7 16-Jul-2012 13:28 1.427 kB 1614216108 to previous | to last
6 16-Jul-2012 13:28 1.432 kB 1614216108 to previous | to last 血液寄生虫学检查 ==> 血液寄生虫检查
5 08-May-2012 10:12 1.433 kB 1614216108 to previous | to last
4 08-May-2012 10:12 1.433 kB 1614216108 to previous | to last
3 24-Apr-2012 11:35 1.421 kB 1614216108 to previous | to last
2 12-Apr-2012 16:02 1.46 kB 1614216108 to previous | to last
1 09-Apr-2012 09:42 1.451 kB 1614216108 to last
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