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This page (revision-20) was last changed on 15-Oct-2013 14:10 by 1614216108

This page was created on 13-Apr-2012 17:35 by UnknownAuthor

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At line 2 changed 3 lines
%%tab-血浆因子Ⅷ抑制物测定
  中文名称:血浆因子Ⅷ抑制物测定  又名:[抗凝血酶III]\\
  英文名称:[plasma factor VIII inhibitor]\\
%%tab-血浆因子Ⅷ抑制物定性测定
  中文名称:血浆因子Ⅷ抑制物定性测定  异名:[抗凝血酶III]\\
  英文名称:[plasma factor VIII inhibitor|Coagulation factor VIII inhibitor]\\
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  定性:阴性
定性:阴性
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%%tab-危急值范围
  无。
/%
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  因子Ⅷ 抑制物定量常用Bethesda方法: 即将患者血浆按不同比例稀释后,与标准正常血浆相混合,37℃温育2小时,测定各混合血浆中的剩余FⅧ:C活性。以剩余的FⅧ:C活性达正常血浆活性的50%时,则患者血浆稀释倍数的倒数为患者血浆中所含的抗体浓度的Bethesda单位(BU)。“Nijemgen” 改良了“ Bethesda ” 试验(1955年引荐,现已广为使用) ,用缓冲液取代正常对照血浆与免疫性缺乏的乏因子Ⅷ血浆的温浴,而正常血浆用于混合温浴时,则用pH7.4咪啶作为缓冲液。\\
  因子Ⅷ抑制物定量常用Bethesda方法: 即将患者血浆按不同比例稀释后,与标准正常血浆相混合,37℃温育2小时,测定各混合血浆中的剩余FⅧ:C活性。以剩余的FⅧ:C活性达正常血浆活性的50%时,则患者血浆稀释倍数的倒数为患者血浆中所含的抗体浓度的Bethesda单位(BU)。\\
  “Nijemgen” 改良了“ Bethesda ” 试验(1955年引荐,现已广为使用) ,用缓冲液取代正常对照血浆与免疫性缺乏的乏因子Ⅷ血浆的温浴,而正常血浆用于混合温浴时,则用pH7.4咪啶作为缓冲液。\\
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  1.采用静脉采血进行检测。静脉采血前要仔细检查针头是否安装牢固,针筒内是否有空气和水分。所用针头应锐利、光滑、通气,针筒不漏气。\\
  2.先用30g/L碘酊棉签自所选静脉穿刺处从内向外、顺时针方向消毒皮肤,待碘酊挥发后,再用75%乙醇棉签以同样方法拭去碘迹。\\
  3.以左手拇指固定静脉穿刺部位下端,右手拇指和中指持注射器针筒,食指固定针头下座,使针头斜面和针筒刻度向上,沿静脉走向使针头与皮肤成30°角斜行快速刺入皮肤,然后以5°角向前穿破静脉壁进入静脉腔。\\
  4.见回血后,将针头顺势探入少许,以免采血时针头滑出;但不可用力深刺,以免造成血肿,同时立即去掉压脉带。针栓只能外抽,不能内推,以免静脉内注入空气形成气栓,造成严重后果。取下注射器针头,将血液沿试管壁缓缓注入抗凝管中,防止溶血和泡沫产生。采得血样分离出血浆。
标本采集:1.采用静脉采血进行检测。静脉采血前要仔细检查针头是否安装牢固,针筒内是否有空气和水分。所用针头应锐利、光滑、通气,针筒不漏气。2.先用30g/L碘酊棉签自所选静脉穿刺处从内向外、顺时针方向消毒皮肤,待碘酊挥发后,再用75%乙醇棉签以同样方法拭去碘迹。3.以左手拇指固定静脉穿刺部位下端,右手拇指和中指持注射器针筒,食指固定针头下座,使针头斜面和针筒刻度向上,沿静脉走向使针头与皮肤成30°角斜行快速刺入皮肤,然后以5°角向前穿破静脉壁进入静脉腔。4.见回血后,将针头顺势探入少许,以免采血时针头滑出;但不可用力深刺,以免造成血肿,同时立即去掉压脉带。针栓只能外抽,不能内推,以免静脉内注入空气形成气栓,造成严重后果。取下注射器针头,将血液沿试管壁缓缓注入抗凝管中,防止溶血和泡沫产生。采得血样分离出血浆。\\
标本运送:采集后立即送到实验室。
Version Date Modified Size Author Changes ... Change note
20 15-Oct-2013 14:10 1.543 kB 1614216108 to previous
19 15-Oct-2013 14:09 1.537 kB 1614216108 to previous | to last Plasma factor VIII inhibitor ==> Coagulation factor VIII inhibitor
18 15-Oct-2013 14:08 1.503 kB 1614216108 to previous | to last 血浆因子VIII抑制物测定 ==> 血浆因子Ⅷ抑制物定性测定
17 30-Jul-2013 14:28 1.503 kB 1614216108 to previous | to last
16 21-Dec-2012 13:52 1.503 kB 1614216108 to previous | to last
15 12-Jul-2012 10:18 1.518 kB 1614216108 to previous | to last
14 12-Jul-2012 10:18 1.574 kB 1614216108 to previous | to last
13 12-Jul-2012 10:17 1.57 kB 1614216108 to previous | to last
12 12-Jul-2012 10:17 1.018 kB 1614216108 to previous | to last
11 03-May-2012 13:23 1.049 kB 1614216108 to previous | to last
10 25-Apr-2012 15:30 1.027 kB 1614216108 to previous | to last
9 24-Apr-2012 10:28 1.024 kB 1614216108 to previous | to last 血浆因子Ⅷ抑制物测定 ==> 血浆因子VIII抑制物测定
8 24-Apr-2012 10:28 1.024 kB 1614216108 to previous | to last
7 23-Apr-2012 10:22 0.991 kB 余志华 to previous | to last
6 16-Apr-2012 12:25 0.965 kB Administrator to previous | to last
5 16-Apr-2012 09:44 0.965 kB Administrator to previous | to last
4 16-Apr-2012 09:44 0.963 kB Administrator to previous | to last
3 16-Apr-2012 08:55 0.969 kB 1614216108 to previous | to last
2 13-Apr-2012 17:38 0.967 kB 1614216108 to previous | to last
1 13-Apr-2012 17:35 0.84 kB UnknownAuthor to last
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