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This page (revision-15) was last changed on 18-Aug-2013 17:32 by 陆颖锋

This page was created on 14-Aug-2013 16:08 by 1614216108

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%%tabbedSection
%%tab-原理
利用游离的鲎三肽与红色偶氮试剂发生反应所形成的红色偶氮蓝复合物
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/%
%%tab-主要仪器
[细菌内毒素动态浊度发检测 ]
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[真菌葡聚糖检测]
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/%
%%tab-参考范围
(也可以选择正常值范围或国际标准）
14-40
[18种氨基酸检测]
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/%
%%tab-科学意义
血浆细菌内毒素水平是革兰氏阴性菌感染的一个重要指标，是监测细菌性(尤其是革兰氏阴性菌)疾病感染的一个重要参数
[MAILD-TOF分子量检测]
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/%
%%tab-样本要求
须使用无热源抗凝采血管采集
[分子量测定1]
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/%
%%tab-收费
30元/位
[分子量测定2]
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/%
%%tab-项目负责人
陆颖锋
/%
/%
[蛋白与代谢组学平台]
[分子量测定3]
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[LC-MSMS鉴定蛋白质-1]
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----
[LC-MSMS鉴定蛋白质-2]
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[细菌内毒素动态浊度发检测]
%%tabbedSection
%%tab-原理
革兰氏阴性菌释放的细菌内毒素能使鲎试剂形成凝胶，在凝胶形成的过程中反应液的浊度增加，利用鲎试验微生物检测系统来记录反应液OD值的变化，OD值上升到某一预设限值所需要的时间与内毒素浓度成负相关，根据标准曲线可以定量检测血浆中的细菌内毒素。
[手动测序鉴定蛋白质1]
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%%tab-主要仪器
exe 808 动态酶标仪
[手动测序鉴定蛋白质2]
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/%
%%tab-参考范围
(也可以选择正常值范围或国际标准）
＜0.1EU/ml
[手动测序鉴定蛋白质3]
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/%
%%tab-科学意义
血浆细菌内毒素水平是革兰氏阴性菌感染的一个重要指标，是监测细菌性(尤其是革兰氏阴性菌)疾病感染的一个重要参数
[糖蛋白质分析]
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/%
%%tab-样本要求
须使用无热源抗凝采血管采集
[LC－MSMS小分子分析]
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/%
%%tab-收费
100/位
[多肽测序（每残基）]
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/%
%%tab-项目负责人
陆颖锋
/%
/%
[蛋白与代谢组学平台 ]
[磷酸化蛋白、突变点、二硫键分析]
[C端序列分析]
[总蛋白含量测定]
[蛋白精确质量测定]
[LC-MS肽指纹图谱]
[LC-MSMS测序]
[数据库下载]
[溶液内蛋白样品制备]
[多肽多维液相分离]
[iTRAQ定量标记（100ug）]
[SILAC定量分析]
[TMT定量标记（100ug）]
[Diart定量标记（100ug）]
[LC-MSMS测序]
[非标记定量蛋白组学]
[蛋白翻译后修饰（磷酸化与糖基化）]
[从头测序De novo]
[普通2DE样品制备]
[2D电泳]
[DIGE电泳]
[胶图分析]
[酶切]
[LC-MS肽指纹图谱]
[LC-MSMS测序]
[蛋白翻译后修饰（磷酸化与糖基化）]
[突变点分析]
[从头测序De novo]
[普通1DE样品制备]
[蛋白1Dgel分离]
[酶切]
[LC-MSMS测序]
[蛋白翻译后修饰（磷酸化与糖基化）]
[非标记定量蛋白组学]
[SILAC定量分析]
[从头测序De novo]
[翻译后修饰富集]
[蛋白翻译后修饰（磷酸化与糖基化）]
[气象色谱与质谱分析]
Version Date Modified Size Author Changes ... Change note
15 18-Aug-2013 17:32 0.836 kB 陆颖锋 to previous
14 18-Aug-2013 17:29 0.837 kB 陆颖锋 to previous | to last
13 16-Aug-2013 17:44 0.839 kB 陆颖锋 to previous | to last
12 16-Aug-2013 17:36 0.837 kB 陆颖锋 to previous | to last
11 16-Aug-2013 17:24 0.08 kB 陆颖锋 to previous | to last
10 16-Aug-2013 17:24 0.08 kB 陆颖锋 to previous | to last
9 15-Aug-2013 21:45 0.072 kB 陆颖锋 to previous | to last
8 15-Aug-2013 21:39 0.054 kB 陆颖锋 to previous | to last
7 15-Aug-2013 21:36 0.745 kB 陆颖锋 to previous | to last
6 15-Aug-2013 21:34 0.76 kB 陆颖锋 to previous | to last
5 15-Aug-2013 21:34 0.313 kB 陆颖锋 to previous | to last
4 15-Aug-2013 21:31 0.706 kB 陆颖锋 to previous | to last
3 15-Aug-2013 21:26 0.314 kB 陆颖锋 to previous | to last
2 15-Aug-2013 21:16 0.014 kB 陆颖锋 to previous | to last
1 14-Aug-2013 16:08 0.012 kB 1614216108 to last
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