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This page (revision-6) was last changed on 14-Oct-2013 15:01 by 1614216108

This page was created on 16-Apr-2012 10:29 by UnknownAuthor

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At line 3 changed 2 lines
　　中文名称：葡萄糖6-磷酸脱氢酶荧光斑点试验 G6PD fluorescent spot test\\
　　英文名称：G6PD fluorescent spot test\\
　　中文名称：葡萄糖6-磷酸脱氢酶荧光斑点试验\\
　　英文名称：[G6PD fluorescent spot test]\\
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G6PD荧光斑点试验根据G6PD可催化G6P（葡萄糖6-磷酸）和NADP+形成6-磷酸葡萄糖酸和NADPH的原理进行检测，后者在长波紫外光（260-365nm）下可发生可见荧光, G6PD活性越强,荧光越强。\\
　　G6PD荧光斑点试验根据G6PD可催化G6P（葡萄糖6-磷酸）和NADP+形成6-磷酸葡萄糖酸和NADPH的原理进行检测，后者在长波紫外光（260-365nm）下可发生可见荧光, G6PD活性越强,荧光越强。\\
__何时检测：__\\
　　需要检查的人群有头晕、头疼、心悸、呼吸困难、腹痛、腰背痛。严重血红蛋白尿者可导致肾功能衰竭症状的人群。
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　　G6PD缺乏的病人10min不出现荧光或仅显示弱荧光(第2斑点和第1斑点比较无明显变化。检查结果有荧光点，葡萄糖-6-磷酸脱氢酶的活性降低。 临床发现贫血外貌、皮肤、巩膜黄染、肝脾轻度肿大或正常。可能患有溶血性贫血。药物反应(如伯氨喹、磺胺毗啶、乙酰苯胺等)、蚕豆病、感染等患者也有可能出现荧光点。
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　　10min时，健康人第2斑点出现强荧光，G6PD缺乏杂合子出现弱荧光，G6PD严重缺乏者无荧光。
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%%tab-医学决定水平
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1. 灵敏度和特异性 本方法直接测定NADPH的量，有较强的特异性。G6PD较稳定，可将吸收标本的滤纸片送检测中心等检测，适用于基层普查。 \\
　　2. 干扰因素 每次试验应用正常标本做阴性对照；贫血严重取血量应加大；在反应液加入1份8.0mmol/L GSSG提高敏感性。\\
1. 灵敏度和特异性 \\
　　本方法直接测定NADPH的量，有较强的特异性。G6PD较稳定，可将吸收标本的滤纸片送检测中心等检测，适用于基层普查。 \\
2. 干扰因素\\
　　每次试验应用正常标本做阴性对照；贫血严重取血量应加大；在反应液加入1份8.0mmol/L GSSG提高敏感性。\\
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　　无特殊要求。\\
　　禁服蚕豆及其制品，停服氧化类药物。试验不合宜人群：血红蛋白<60g/L的患者\\
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[红细胞酶缺陷]
Version Date Modified Size Author Changes ... Change note
6 14-Oct-2013 15:01 0.82 kB 1614216108 to previous
5 13-Jul-2012 10:35 0.867 kB 1614216108 to previous | to last
4 13-Jul-2012 10:35 0.658 kB 1614216108 to previous | to last
3 03-May-2012 15:44 0.687 kB 1614216108 to previous | to last
2 23-Apr-2012 11:35 0.674 kB 1614216108 to previous | to last
1 16-Apr-2012 10:29 0.564 kB UnknownAuthor to last
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