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%%tab-原理
荧光素酶可以催化luciferin氧化成oxyluciferin,在luciferin氧化的过程中,会发出生物荧光(bioluminescence)。然后可以通过荧光测定仪也称化学发光仪(luminometer)测定luciferin氧化过程中释放的生物荧光。
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%%tab-主要仪器
GloMax™ 96 Microplate luminometer
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%%tab-参考范围
3X10-21摩尔萤光素酶;线性响应范围大于9个数量
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%%tab-科学意义
荧光素和荧光素酶这一生物发光体系,可以极其灵敏、高效地检测基因的表达。
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%%tab-样本要求
细胞培养上清,<100微升
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%%tab-收费
200元/板(自带试剂),50元/孔(提供试剂)
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%%tab-项目负责人
朱海红
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[细胞生物学平台]
Version Date Modified Size Author Changes ... Change note
1 20-Aug-2013 10:16 0.429 kB 吴珊珊
« This page (revision-1) was last changed on 20-Aug-2013 10:16 by 吴珊珊