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This page (revision-12) was last changed on 14-Oct-2013 16:25 by 1614216108

This page was created on 16-Apr-2012 13:03 by UnknownAuthor

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%%tab-肽链裂解试验（聚丙烯酰胺凝胶电泳）
　　中文名称：肽链裂解试验（聚丙烯酰胺凝胶电泳）\\
　　英文名称:\\
%%tab-聚丙烯酰胺凝胶电泳
　　中文名称：聚丙烯酰胺凝胶电泳；又名：[肽链裂解试验]\\
　　英文名称:［polyacrylamide gel electrophoresis，PAGE]\\
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　　本试验可对血红蛋白肽链异常做出初步判断，比一般的醋纤膜电泳敏感，但应进一步进行血红蛋白一级结构的分析，才能进行异常血红蛋白的确诊。本试验要求条件较高，操作繁琐。一般实验室多用醋纤膜电泳进行初步筛检。\\
　　本试验可对血红蛋白肽链异常做出初步判断，比一般的醋纤膜电泳敏感，但应进一步进行血红蛋白一级结构的分析，才能进行异常血红蛋白的确诊。试验要求条件较高，操作繁琐，一般实验室多用醋纤膜电泳进行初步筛检。\\
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　　肝素抗凝全血标本。\\
　　肝素抗凝全血标本。将样品按0.5–1 mg/mL加样品溶解液，溶解后，将其转移到带塞的小离心管中，轻轻盖上盖子(不要塞紧，以免加热时迸出)，在100℃沸水浴中加热3min，取出冷却后加样。样品中应含一定浓度的蔗糖溶液，目的是用以防止样品因对流而被稀释。 \\
Version Date Modified Size Author Changes ... Change note
12 14-Oct-2013 16:25 0.027 kB 1614216108 to previous
11 14-Oct-2013 15:22 0.811 kB 1614216108 to previous | to last
10 13-Jul-2012 11:15 0.808 kB 1614216108 to previous | to last
9 13-Jul-2012 11:15 0.808 kB 1614216108 to previous | to last
8 13-Jul-2012 11:15 0.811 kB 1614216108 to previous | to last 肽链裂解试验 ==> 聚丙烯酰胺凝胶电泳
7 13-Jul-2012 11:14 0.808 kB 1614216108 to previous | to last 肽链裂解试验 ==> 聚丙烯酰胺凝胶电泳
6 13-Jul-2012 11:14 0.808 kB 1614216108 to previous | to last
5 13-Jul-2012 11:14 0.658 kB 1614216108 to previous | to last
4 03-May-2012 16:09 0.669 kB 1614216108 to previous | to last
3 23-Apr-2012 09:54 0.658 kB 1614216108 to previous | to last 肽链裂解试验（聚丙烯酰胺凝胶电泳） ==> 肽链裂解试验
2 23-Apr-2012 09:52 0.658 kB 1614216108 to previous | to last
1 16-Apr-2012 13:03 0.623 kB UnknownAuthor to last
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