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This page (revision-7) was last changed on 14-Oct-2013 13:59 by 1614216108

This page was created on 16-Apr-2012 12:53 by UnknownAuthor

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At line 6 changed one line
红细胞在37℃孵育48h后,观察红细胞的溶血情况,称为自身溶血试验。红细胞在孵育时发生溶血增加的主要原因有:膜异常引起钠内流倾向明显增加,钠泵功能增强,ATP消耗过多;或糖酵解途径酶缺乏所引起ATP生成不足。这些原因都可导致钠泵作用减弱,红细胞内钠离子和水积聚过多,溶血增加。在孵育时加入葡萄糖或ATP作为纠正物,观察溶血可否有一定的纠正,称为红细胞自身溶血试验的纠正试验,进行纠正试验有助于鉴别贫血的类型。\\
  红细胞在37℃孵育48h后,观察红细胞的溶血情况,称为自身溶血试验。红细胞在孵育时发生溶血增加的主要原因有:膜异常引起钠内流倾向明显增加,钠泵功能增强,ATP消耗过多;或糖酵解途径酶缺乏所引起ATP生成不足。这些原因都可导致钠泵作用减弱,红细胞内钠离子和水积聚过多,溶血增加。在孵育时加入葡萄糖或ATP作为纠正物,观察溶血可否有一定的纠正,称为红细胞自身溶血试验的纠正试验,进行纠正试验有助于鉴别贫血的类型。\\
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  1.遗传性球形红细胞增多症溶血率增加,加入葡萄糖或ATP后可明显纠正。\\
  2.G6PD缺乏症等戊糖旁路代谢缺乏的病人溶血率增加,能被葡萄糖纠正。\\
  3.丙酮酸激酶缺乏症时不能利用葡萄糖产生ATP,其溶血率明显增加,加葡萄糖不能纠正,加ATP可纠正。\\
  4.获得性溶血性贫血或自身免疫性溶血时,试验结果常各有不同,对诊断意义不大。\\
1.遗传性球形红细胞增多症溶血率增加,加入葡萄糖或ATP后可明显纠正。\\
2.G6PD缺乏症等戊糖旁路代谢缺乏的病人溶血率增加,能被葡萄糖纠正。\\
3.丙酮酸激酶缺乏症时不能利用葡萄糖产生ATP,其溶血率明显增加,加葡萄糖不能纠正,加ATP可纠正。\\
4.获得性溶血性贫血或自身免疫性溶血时,试验结果常各有不同,对诊断意义不大。\\
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  健康人红细胞孵育48小时,不加纠正物的溶血率0.25%~4.0%,加葡萄糖的溶血率0.1%~0.6%,加ATP纠正物的溶血率0.1%~0.8%。
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Version Date Modified Size Author Changes ... Change note
7 14-Oct-2013 13:59 1.101 kB 1614216108 to previous
6 14-Oct-2013 13:58 1.101 kB 1614216108 to previous | to last
5 12-Jul-2012 16:18 1.148 kB 1614216108 to previous | to last
4 12-Jul-2012 16:18 0.863 kB 1614216108 to previous | to last
3 03-May-2012 16:13 0.883 kB 1614216108 to previous | to last
2 23-Apr-2012 10:00 0.851 kB 1614216108 to previous | to last
1 16-Apr-2012 12:53 0.778 kB UnknownAuthor to last
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