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This page (revision-7) was last changed on 14-Oct-2013 14:57 by 1614216108

This page was created on 16-Apr-2012 14:18 by UnknownAuthor

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丙酮酸激酶荧光斑点和活性测定是在二磷酸腺苷（ADP）存在条件下丙酮酸激酶（PK）催化磷酸烯醇丙酮酸()转化成丙酮酸，在辅酶Ⅰ还原型(NADH)存在情况下，丙酮酸被LDH转化为乳酸，若标记荧光于NADH上，此时有荧光的NADH变为无荧光的NAD＋。\\
　　丙酮酸激酶荧光斑点和活性测定是在二磷酸腺苷（ADP）存在条件下丙酮酸激酶（PK）催化磷酸烯醇丙酮酸()转化成丙酮酸，在辅酶Ⅰ还原型(NADH)存在情况下，丙酮酸被LDH转化为乳酸，若标记荧光于NADH上，此时有荧光的NADH变为无荧光的NAD＋。\\
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　　健康人丙酮酸激酶活性斑点在25分钟之内消失，酶活性（15.0±1.99）U/gHb。
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在试验中因白细胞、血小板、网织红细胞均使结果增高，故应尽量除去。同时试剂的pH值、温度要准确。\\
　　在试验中因白细胞、血小板、网织红细胞均使结果增高，故应尽量除去。同时试剂的pH值、温度要准确。\\
Version Date Modified Size Author Changes ... Change note
7 14-Oct-2013 14:57 0.788 kB 1614216108 to previous
6 13-Jul-2012 10:07 0.836 kB 1614216108 to previous | to last
5 13-Jul-2012 10:07 0.591 kB 1614216108 to previous | to last
4 03-May-2012 15:44 0.611 kB 1614216108 to previous | to last
3 20-Apr-2012 16:10 0.579 kB 1614216108 to previous | to last 红细胞丙酮酸激酶测定(PK) ==> 红细胞丙酮酸激酶测定
2 20-Apr-2012 16:09 0.579 kB 1614216108 to previous | to last
1 16-Apr-2012 14:18 0.53 kB UnknownAuthor to last
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