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This page (revision-6) was last changed on 16-Oct-2013 14:46 by 1614216108

This page was created on 16-Apr-2012 15:46 by UnknownAuthor

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%%tab-硝基四氮唑蓝（NBT）还原试验
　　中文名称：硝基四氮唑蓝（NBT）还原试验 \\
　　英文名称：\\
%%tab-硝基四氮唑蓝还原试验
　　中文名称：硝基四氮唑蓝还原试验 \\
　　英文名称：[Nitroubluetetrazolium reduction test]\\
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二、检测方法\\
　　经典的定性测量法是在盖玻片上滴一滴血标本，置容器中加盖，在湿环境下放置25min，此时中性粒细胞即粘附于玻片上。用生理盐水轻轻冲洗去血凝块，用滤纸吸去多余水分，加1滴NBT试剂（0.28%NBT生理盐水溶液0.6ml，血清0.5ml和生理盐水0.3ml的混合液），覆盖后37摄氏度湿润环境下反应20min。取出盖玻片，干燥、固定、染色，在显微镜下计数细胞内有蓝色甲糌颗粒的中性粒细胞所占比例。\\
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%%tab-医学决定水平
　　无\\
%%tab-方法学评价
　　经典的定性测量法是在盖玻片上滴一滴血标本，置容器中加盖，在湿环境下放置25min，此时中性粒细胞即粘附于玻片上。用生理盐水轻轻冲洗去血凝块，用滤纸吸去多余水分，加1滴NBT试剂（0.28%NBT生理盐水溶液0.6ml，血清0.5ml和生理盐水0.3ml的混合液），覆盖后37摄氏度湿润环境下反应20min。取出盖玻片，干燥、固定、染色，在显微镜下计数细胞内有蓝色甲糌颗粒的中性粒细胞所占比例。\\
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%%tab-危急值范围
　　无\\
/%
/%
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　　\\
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[细胞免疫指标]
Version Date Modified Size Author Changes ... Change note
6 16-Oct-2013 14:46 0.668 kB 1614216108 to previous
5 04-May-2012 16:06 0.689 kB 1614216108 to previous | to last
4 04-May-2012 16:06 0.7 kB 1614216108 to previous | to last
3 20-Apr-2012 14:43 0.679 kB 1614216108 to previous | to last 硝基四氮唑蓝（NBT）还原试验 ==> 硝基四氮唑蓝还原试验
2 20-Apr-2012 14:43 0.679 kB 1614216108 to previous | to last
1 16-Apr-2012 15:46 0.683 kB UnknownAuthor to last
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