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This page (revision-3) was last changed on 11-Oct-2013 14:59 by 1614216108

This page was created on 20-Dec-2012 13:37 by 1614216108

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泪液溶菌酶浓度降低见于干性角结膜炎及疱疹性角膜炎。
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(1)沉淀反应免疫比浊法：当抗体过剩时，抗原、抗体最适比值浓度范围大致呈直线。应用紫外波长(340nm)可测定抗原抗体复合物浊度。大多数光电比色计，分光光度计及自动生化分析仪均可用于比浊。\\
(2)乳胶颗粒被动凝集免疫比浊法：将抗体致敏在乳胶颗粒上，加上抗原时，致敏的乳胶颗粒与抗原相结合，形成大小不等的凝集块。凝集颗粒小时，光可通过；凝集颗粒大时，光散乱，透过光减少。
1.沉淀反应免疫比浊法：当抗体过剩时，抗原、抗体最适比值浓度范围大致呈直线。应用紫外波长(340nm)可测定抗原抗体复合物浊度。大多数光电比色计，分光光度计及自动生化分析仪均可用于比浊。\\
2.乳胶颗粒被动凝集免疫比浊法：将抗体致敏在乳胶颗粒上，加上抗原时，致敏的乳胶颗粒与抗原相结合，形成大小不等的凝集块。凝集颗粒小时，光可通过；凝集颗粒大时，光散乱，透过光减少。
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%%tab-注意事项
1.透射比浊时，应采用颗粒吸收最强烈的波长，否则将影响曲线斜率。\\
2.应保证检测仪器的良好性能，测定仪器光学系统调换零件时，应重新绘制比浊法的曲线，否则容易引起误差。\\
3.比浊法易受外界条件的影响，因此，试验条件、试剂浓度、用量等要求一致，混悬液中颗粒大小要均匀，重现性好，否则将影响结果。
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Version Date Modified Size Author Changes ... Change note
3 11-Oct-2013 14:59 0.78 kB 1614216108 to previous
2 20-Dec-2012 13:44 0.786 kB 1614216108 to previous | to last
1 20-Dec-2012 13:37 0.596 kB 1614216108 to last
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