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This page (revision-67) was last changed on 23-Aug-2013 15:22 by 曹清毅

This page was created on 14-Aug-2013 15:07 by 1614216108

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革兰氏阴性菌释放的细菌内毒素能使鲎试剂形成凝胶，在凝胶形成的过程中反应液的浊度增加，利用鲎试验微生物检测系统来记录反应液OD值的变化，OD值上升到某一预设限值所需要的时间与内毒素浓度成负相关，根据标准曲线可以定量检测血浆中的细菌内毒素。
采用微囊化技术和转瓶培养技术相结合，进行细胞大规模培养，获取亿万级数量的细胞。
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细胞转瓶机
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(也可以选择正常值范围或国际标准）
＜0.1EU/ml
1亿至10万亿细胞数量。
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/%
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血浆细菌内毒素水平是革兰氏阴性菌感染的一个重要指标，是监测细菌性(尤其是革兰氏阴性菌)疾病感染的一个重要参数
细胞经转瓶培养后，细胞活率越高越好，一般要在90%以上，活力偏低时说明细胞活力差不利于后续实验进行。
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须使用无热源抗凝采血管采集
需无菌操作。
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100元/位
4510元/转瓶
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陆颖锋
潘小平
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[细菌内毒素动态浊度发检测]
Version Date Modified Size Author Changes ... Change note
67 23-Aug-2013 15:22 0.186 kB 曹清毅 to previous
66 21-Aug-2013 16:36 0.186 kB 1614216108 to previous | to last
65 21-Aug-2013 16:09 0.245 kB 6A18P3 to previous | to last
64 21-Aug-2013 16:07 0.245 kB 6A18P3 to previous | to last
63 21-Aug-2013 16:07 0.246 kB 6A18P3 to previous | to last
62 21-Aug-2013 15:59 0.293 kB 6A18P3 to previous | to last
61 21-Aug-2013 15:50 0.266 kB 6A18P3 to previous | to last
« This page (revision-67) was last changed on 23-Aug-2013 15:22 by 曹清毅