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This page (revision-67) was last changed on 23-Aug-2013 15:22 by 曹清毅

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通过逆转录PCR（RT-PCR）方法扩增HIV-1 pol基因的特定区域，并对PCR产物进行测序，将所测序列与数据库中的参考序列或共享序列比较，判断是否出现耐药相关的基因突变，并根据HIV-1耐药基因型解释系统的规则来评判对特定药物的耐药程度。
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Eppendorf PCR仪和ABI基因测序仪
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分基因区(蛋白酶（PRO）和逆转录酶（RT）)报告耐药相关的基因突变，基因突变以“字母－数字－字母”的书写方式来表示，第一个字母代表野生型病毒株特定密码子处的氨基酸，第二个字母代表在特定密码子处替换了的氨基酸。根据HIV-1耐药基因型解释系统报告耐药程度：敏感（S）、潜在耐药（P）、低度耐药（L）、中度耐药（I）和高度耐药（H）五个水平。
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用于个体患者的耐药检测。在抗病毒治疗前进行耐药检测，可指导临床医生制定抗病毒治疗方案，保证抗病毒治疗的效果；在抗病毒治疗过程中进行耐药检测，可指导临床医生分析治疗失败的原因，并制定补救治疗方案。
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EDTA抗凝全血5 mL,要求感染者血浆病毒载量≥1000拷贝/毫升或国际单位。
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1800元
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姚航平
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[P3实验室平台]
[生物信息学平台]
Version Date Modified Size Author Changes ... Change note
67 23-Aug-2013 15:22 0.186 kB 曹清毅 to previous
66 21-Aug-2013 16:36 0.186 kB 1614216108 to previous | to last
65 21-Aug-2013 16:09 0.245 kB 6A18P3 to previous | to last
64 21-Aug-2013 16:07 0.245 kB 6A18P3 to previous | to last
63 21-Aug-2013 16:07 0.246 kB 6A18P3 to previous | to last
62 21-Aug-2013 15:59 0.293 kB 6A18P3 to previous | to last
61 21-Aug-2013 15:50 0.266 kB 6A18P3 to previous | to last
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