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This page (revision-67) was last changed on 23-Aug-2013 15:22 by 曹清毅

This page was created on 14-Aug-2013 15:07 by 1614216108

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采用Ficoll密度梯度离心法分离大鼠骨髓中的单核细胞。Ficoll是蔗糖的多聚体，呈中性，平均分子量为400，000，当密度为1.2g／ml，未超出正常生理性渗透压，也不穿过生物膜。红细胞、粒细胞比重大，离心后沉于管底；淋巴细胞和单核细胞的比重小于或等于分层液比重，离心后漂浮于分层液的液面上，也可有少部分细胞悬浮在分层液中。吸取分层液液面的细胞，就可从外周血中分离到单个核细胞。单核细胞贴壁培养形成克隆后经传代提纯得到骨髓干细胞。/%
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低温离心机；培养箱；
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得到的细胞经流式检测CD29和CD90表达阳性，CD34和CD45表达阴性。
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得到骨髓干细胞以用于诱导分化为肝脏、脂肪、成骨等细胞，或移植进行干细胞治疗
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大鼠60-120g
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105元/次：
DMEM(50ml,40元/250ml)；胎牛血清（5ml,3000元/500ml）；培养瓶2个（10元/个）；FICOLL（10ml，1600元/500ml），15ml离心管（3个，5元/个）
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辛娇娇 杨金凤 俞炯
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[人工肝平台--分离大鼠骨髓干细胞]
[人工肝平台]
Version Date Modified Size Author Changes ... Change note
67 23-Aug-2013 15:22 0.186 kB 曹清毅 to previous
66 21-Aug-2013 16:36 0.186 kB 1614216108 to previous | to last
65 21-Aug-2013 16:09 0.245 kB 6A18P3 to previous | to last
64 21-Aug-2013 16:07 0.245 kB 6A18P3 to previous | to last
63 21-Aug-2013 16:07 0.246 kB 6A18P3 to previous | to last
62 21-Aug-2013 15:59 0.293 kB 6A18P3 to previous | to last
61 21-Aug-2013 15:50 0.266 kB 6A18P3 to previous | to last
« This page (revision-67) was last changed on 23-Aug-2013 15:22 by 曹清毅