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This page (revision-5) was last changed on 16-Oct-2013 14:40 by 1614216108

This page was created on 17-Apr-2012 14:34 by UnknownAuthor

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At line 7 removed 9 lines
二、 检测方法\\
  巨噬细胞对颗粒性抗原物质具有很强的吞噬功能,常用鸡红细胞(CRBC)、白色念珠菌、酵母细胞等作为吞噬颗粒,用斑螯敷贴法收集人巨噬细胞或从腹腔渗出液获得鼠巨噬细胞。将巨噬细胞和鸡红细胞悬液在体外混合、温育、涂片、染色,油镜下观察计数,通过计算吞噬率和吞噬指数来反应巨噬细胞的吞噬功能,并通过观察鸡红细胞的消化程度,判断巨噬细胞的消化功能。\\
按下述公式计算吞噬百分率和吞噬指数,记录消化程度。\\
吞噬率%=吞噬鸡红细胞的巨噬细胞数/200×100%\\
吞噬指数=巨噬细胞吞噬的鸡红细胞总数/200\\
消化程度分3级:\\
Ⅰ级:未消化:CRBC核清晰,浅紫红色,胞质浅红或黄绿色。\\
Ⅱ级:轻度消化:CRBC核模糊,肿胀,紫蓝色,胞质浅黄绿色。\\
Ⅲ级:完全消化:CRBC核完全溶解,染色极淡,胞质中见空泡。\\
At line 9 added one line
  恶性肿瘤患者的吞噬率和吞噬指数均降低
At line 20 added 9 lines
%%tab-方法学评价
  巨噬细胞对颗粒性抗原物质具有很强的吞噬功能,常用鸡红细胞(CRBC)、白色念珠菌、酵母细胞等作为吞噬颗粒,用斑螯敷贴法收集人巨噬细胞或从腹腔渗出液获得鼠巨噬细胞。将巨噬细胞和鸡红细胞悬液在体外混合、温育、涂片、染色,油镜下观察计数,通过计算吞噬率和吞噬指数来反应巨噬细胞的吞噬功能,并通过观察鸡红细胞的消化程度,判断巨噬细胞的消化功能。\\
  按下述公式计算吞噬百分率和吞噬指数,记录消化程度。\\
  吞噬率%=吞噬鸡红细胞的巨噬细胞数/200×100%\\
  吞噬指数=巨噬细胞吞噬的鸡红细胞总数/200\\
消化程度分3级:\\
Ⅰ级:未消化:CRBC核清晰,浅紫红色,胞质浅红或黄绿色。\\
Ⅱ级:轻度消化:CRBC核模糊,肿胀,紫蓝色,胞质浅黄绿色。\\
Ⅲ级:完全消化:CRBC核完全溶解,染色极淡,胞质中见空泡。\\
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  无特殊要求\\
\\
  无特殊要求
Version Date Modified Size Author Changes ... Change note
5 16-Oct-2013 14:40 0.782 kB 1614216108 to previous
4 04-May-2012 16:03 0.831 kB 1614216108 to previous | to last
3 04-May-2012 16:03 0.842 kB 1614216108 to previous | to last
2 19-Apr-2012 13:29 0.821 kB 1614216108 to previous | to last
1 17-Apr-2012 14:34 0.797 kB UnknownAuthor to last
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