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This page (revision-8) was last changed on 21-Dec-2012 10:55 by 1614216108

This page was created on 17-Apr-2012 10:36 by UnknownAuthor

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1.热放散：①取具有相应抗原红细胞的抗凝血离心、吸去血浆，加大量生理盐水洗涤3次，离心取压积红细胞备用；②取受检者血清5ml，加上述压积红细胞1ml。若查IgG抗体混匀后放37℃水浴1h，若查IgM抗体混匀后放4℃冰箱1h，在此期间混合5—6次；③取出后离心，吸出上清液弃去，用生理盐水洗涤红细胞3次，离心去上清液、留压积红细胞；④将预热56℃的AB型(经选择无抗A、抗B、抗H、抗Rh等抗体者)血清或生理盐水加入等量压积红细胞内，放入56℃水浴中不断摇动7min；⑤取出后立即以3000r/min离心5min(离心管的套管内加56℃温水保温)，离心后樱红色的上清液即为放散液。\\
2.乙醚放散试验：①红细胞处理同加热放散试验操作方法中的①～③。②取试管1支，将洗涤过的1份红细胞加1份生理盐水、2份乙醚，用玻璃纸盖住管口，手指压住，用力上下晃动10min，以 3000r/min离心5min，吸取底部液体即为放散液(一般分3层：上层为乙醚，中层为红细胞基质、下层为放散液)，若混浊可再离心1次取上清液。③将放散液置37℃水浴中30min，促使乙醚挥发。④用放散液与相应抗原作酶法或抗人球蛋白法测抗体。
1.热放散\\
①取具有相应抗原红细胞的抗凝血离心、吸去血浆，加大量生理盐水洗涤3次，离心取压积红细胞备用；②取受检者血清5ml，加上述压积红细胞1ml。若查IgG抗体混匀后放37℃水浴1h，若查IgM抗体混匀后放4℃冰箱1h，在此期间混合5—6次；③取出后离心，吸出上清液弃去，用生理盐水洗涤红细胞3次，离心去上清液、留压积红细胞；④将预热56℃的AB型(经选择无抗A、抗B、抗H、抗Rh等抗体者)血清或生理盐水加入等量压积红细胞内，放入56℃水浴中不断摇动7min；⑤取出后立即以3000r/min离心5min(离心管的套管内加56℃温水保温)，离心后樱红色的上清液即为放散液。\\
2.乙醚放散试验\\
①红细胞处理同加热放散试验操作方法中的①～③。②取试管1支，将洗涤过的1份红细胞加1份生理盐水、2份乙醚，用玻璃纸盖住管口，手指压住，用力上下晃动10min，以 3000r/min离心5min，吸取底部液体即为放散液(一般分3层：上层为乙醚，中层为红细胞基质、下层为放散液)，若混浊可再离心1次取上清液。③将放散液置37℃水浴中30min，促使乙醚挥发。④用放散液与相应抗原作酶法或抗人球蛋白法测抗体。
Version Date Modified Size Author Changes ... Change note
8 21-Dec-2012 10:55 1.237 kB 1614216108 to previous
7 21-Dec-2012 10:54 1.231 kB 1614216108 to previous | to last
6 03-Dec-2012 12:13 1.312 kB 1614216108 to previous | to last 血库 ==> 血型、输血及人类组织相关性抗原检测
5 03-Dec-2012 12:13 1.297 kB 1614216108 to previous | to last
4 13-Jul-2012 17:29 0.702 kB 1614216108 to previous | to last
3 03-May-2012 12:18 0.712 kB 1614216108 to previous | to last
2 20-Apr-2012 10:40 0.696 kB 1614216108 to previous | to last
1 17-Apr-2012 10:36 0.656 kB UnknownAuthor to last
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