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This page (revision-4) was last changed on 22-Aug-2013 14:35 by 朱海红

This page was created on 20-Aug-2013 09:25 by 吴珊珊

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At line 3 changed one line
本试剂盒应用双抗体夹心法测定标本中人白介素1(IL-1)水平。用纯化的人白介素6(IL-1)抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入白介素1(IL-1),再与HRP标记的白介素1(IL-1)抗体结合,形成抗体-抗原-酶标抗体复合物,经过彻底洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的白介素1(IL-1)呈正相关。用酶标仪在450nm波长下测定吸光度(OD值),通过标准曲线计算样品中人白介素1(IL-1)浓度。
本试剂盒应用双抗体夹心法测定标本中人白介素1(IL-1)水平。用纯化的人白介素1(IL-1)抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入白介素1(IL-1),再与HRP标记的白介素1(IL-1)抗体结合,形成抗体-抗原-酶标抗体复合物,经过彻底洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的白介素1(IL-1)呈正相关。用酶标仪在450nm波长下测定吸光度(OD值),通过标准曲线计算样品中人白介素1(IL-1)浓度。
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bio-rad酶标仪
[bio-rad酶标仪]
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200元/人/指标
200元/样本/指标
Version Date Modified Size Author Changes ... Change note
4 22-Aug-2013 14:35 0.709 kB 朱海红 to previous
3 20-Aug-2013 10:21 0.708 kB 何前锋 to previous | to last
2 20-Aug-2013 09:30 0.706 kB 吴珊珊 to previous | to last
1 20-Aug-2013 09:25 0.706 kB 吴珊珊 to last
« This page (revision-4) was last changed on 22-Aug-2013 14:35 by 朱海红