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This page (revision-54) was last changed on 22-Aug-2013 17:53 by Administrator

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[MTT实验]\\
[平台项目编写模板]\\
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MTT法是一种定量检测活细胞存活和生长的方法。活细胞线粒体中的琥珀酸脱氢酶能使外源性MTT被还原为难溶的蓝紫色结晶物并在细胞中沉积，将细胞中的紫色结晶物溶解后，在550 nm-690 nm处测定光吸收值，可间接反映活细胞数量。
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酶标仪
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依据标本情况
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在一定细胞范围内，结晶物的形成量与细胞数成正比，吸光值高则细胞数量多。数值过高或者过低都无法保证MTT结晶形成量与细胞数之间的线性关系。
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细胞数目在10^2-10^6之间
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1500元/周期/孔板
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张艳红
Version Date Modified Size Author Changes ... Change note
54 22-Aug-2013 17:53 0.353 kB Administrator to previous 人工肝研究平台 ==> 人工肝平台
53 22-Aug-2013 17:49 0.353 kB 1614216108 to previous | to last 人工肝平台 ==> 人工肝研究平台
52 22-Aug-2013 17:49 0.353 kB 1614216108 to previous | to last 人工肝研究平台 ==> 人工肝平台
51 21-Aug-2013 14:20 0.353 kB 1614216108 to previous | to last
50 21-Aug-2013 14:18 0.366 kB 1614216108 to previous | to last Hiseq 2000测序 ==> 平台项目编写模板
49 21-Aug-2013 10:38 0.357 kB qinnan to previous | to last 平台项目编写模板 ==> Hiseq 2000测序
48 18-Aug-2013 19:55 0.353 kB 人工肝 to previous | to last
47 18-Aug-2013 19:55 0.354 kB 人工肝 to previous | to last
46 18-Aug-2013 19:49 0.326 kB 人工肝 to previous | to last
45 18-Aug-2013 19:46 0.312 kB 人工肝 to previous | to last
44 18-Aug-2013 19:42 0.284 kB 人工肝 to previous | to last
43 18-Aug-2013 19:38 0.27 kB 人工肝 to previous | to last
42 18-Aug-2013 19:37 0.257 kB 人工肝 to previous | to last
41 18-Aug-2013 19:36 0.255 kB 人工肝 to previous | to last
« This page (revision-54) was last changed on 22-Aug-2013 17:53 by Administrator