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This page (revision-54) was last changed on 22-Aug-2013 17:53 by Administrator

This page was created on 14-Aug-2013 15:03 by 1614216108

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[MTT实验]\\
%%tabbedSection
%%tab-原理
MTT法是一种定量检测活细胞存活和生长的方法。活细胞线粒体中的琥珀酸脱氢酶能使外源性MTT被还原为难溶的蓝紫色结晶物并在细胞中沉积，将细胞中的紫色结晶物溶解后，在550 nm-690 nm处测定光吸收值，可间接反映活细胞数量。
[平台项目编写模板]
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/%
%%tab-主要仪器
酶标仪
[MTT实验]
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/%
%%tab-参考范围
依据标本情况
[微囊制备]
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/%
%%tab-科学意义
在一定细胞范围内，结晶物的形成量与细胞数成正比，吸光值高则细胞数量多。数值过高或者过低都无法保证MTT结晶形成量与细胞数之间的线性关系。
[分离大鼠骨髓干细胞]
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/%
%%tab-样本要求
细胞数目在10^2-10^6之间
[分离人骨髓干细胞]
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/%
%%tab-收费
1500元/周期/孔板
[大鼠肝细胞分离与卵圆细胞分离]
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[小鼠支架移植]
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/%
%%tab-项目负责人
张艳红
/%
/%
[人工肝平台]
[免疫组化]
[细胞存活率分析仪]
[大规模转瓶细胞培养系统]
[Western Blot]
[RT-PCR]
[总RNA抽提]
[肝细胞灌流分离]
[琼脂糖核酸电泳]
[胶回收纯化DNA]
[大肠杆菌质粒的提取]
[ELISA]
[细胞程序降温仪]
[荧光倒置显微镜]
[原代猪肝细胞分离]
[基于永生化人肝细胞漏斗型生物反应器人工肝治疗]
[猪肝衰竭模型制备]
[基于原代猪肝细胞漏斗型生物反应器人工肝治疗]
Version Date Modified Size Author Changes ... Change note
54 22-Aug-2013 17:53 0.353 kB Administrator to previous 人工肝研究平台 ==> 人工肝平台
53 22-Aug-2013 17:49 0.353 kB 1614216108 to previous | to last 人工肝平台 ==> 人工肝研究平台
52 22-Aug-2013 17:49 0.353 kB 1614216108 to previous | to last 人工肝研究平台 ==> 人工肝平台
51 21-Aug-2013 14:20 0.353 kB 1614216108 to previous | to last
50 21-Aug-2013 14:18 0.366 kB 1614216108 to previous | to last Hiseq 2000测序 ==> 平台项目编写模板
49 21-Aug-2013 10:38 0.357 kB qinnan to previous | to last 平台项目编写模板 ==> Hiseq 2000测序
48 18-Aug-2013 19:55 0.353 kB 人工肝 to previous | to last
47 18-Aug-2013 19:55 0.354 kB 人工肝 to previous | to last
46 18-Aug-2013 19:49 0.326 kB 人工肝 to previous | to last
45 18-Aug-2013 19:46 0.312 kB 人工肝 to previous | to last
44 18-Aug-2013 19:42 0.284 kB 人工肝 to previous | to last
43 18-Aug-2013 19:38 0.27 kB 人工肝 to previous | to last
42 18-Aug-2013 19:37 0.257 kB 人工肝 to previous | to last
41 18-Aug-2013 19:36 0.255 kB 人工肝 to previous | to last
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