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This page (revision-54) was last changed on 22-Aug-2013 17:53 by Administrator

This page was created on 14-Aug-2013 15:03 by 1614216108

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[平台项目编写模板]
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[MTT实验]
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[微囊制备]
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[分离大鼠骨髓干细胞]
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%%tabbedSection
%%tab-原理
[分离人骨髓干细胞]
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采用Ficoll密度梯度离心法分离大鼠骨髓中的单核细胞。Ficoll是蔗糖的多聚体，呈中性，平均分子量为400，000，当密度为1.2g／ml，未超出正常生理性渗透压，也不穿过生物膜。红细胞、粒细胞比重大，离心后沉于管底；淋巴细胞和单核细胞的比重小于或等于分层液比重，离心后漂浮于分层液的液面上，也可有少部分细胞悬浮在分层液中。吸取分层液液面的细胞，就可从外周血中分离到单个核细胞。单核细胞贴壁培养形成克隆后经传代提纯得到骨髓干细胞。/%
%%tab-主要仪器
低温离心机；培养箱；
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%%tab-参考范围
得到的细胞经流式检测CD29和CD90表达阳性，CD34和CD45表达阴性。
[大鼠肝细胞分离与卵圆细胞分离]
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%%tab-临床意义
得到骨髓干细胞以用于诱导分化为肝脏、脂肪、成骨等细胞，或移植进行干细胞治疗
/%
%%tab-样本要求
大鼠60-120g
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%%tab-收费
105元/次：
DMEM(50ml,40元/250ml)；胎牛血清（5ml,3000元/500ml）；培养瓶2个（10元/个）；FICOLL（10ml，1600元/500ml），15ml离心管（3个，5元/个）
[小鼠支架移植]
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%%tab-项目负责人
辛娇娇 杨金凤 俞炯
/%
/%
[人工肝平台--分离大鼠骨髓干细胞]
[免疫组化]
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[细胞存活率分析仪]
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--人工肝, 16-八月-2013 08:57
[大规模转瓶细胞培养系统]
[Western Blot]
[RT-PCR]
[总RNA抽提]
[肝细胞灌流分离]
[琼脂糖核酸电泳]
[胶回收纯化DNA]
[大肠杆菌质粒的提取]
[ELISA]
[细胞程序降温仪]
[荧光倒置显微镜]
[原代猪肝细胞分离]
[基于永生化人肝细胞漏斗型生物反应器人工肝治疗]
[猪肝衰竭模型制备]
[基于原代猪肝细胞漏斗型生物反应器人工肝治疗]
Version Date Modified Size Author Changes ... Change note
54 22-Aug-2013 17:53 0.353 kB Administrator to previous 人工肝研究平台 ==> 人工肝平台
53 22-Aug-2013 17:49 0.353 kB 1614216108 to previous | to last 人工肝平台 ==> 人工肝研究平台
52 22-Aug-2013 17:49 0.353 kB 1614216108 to previous | to last 人工肝研究平台 ==> 人工肝平台
51 21-Aug-2013 14:20 0.353 kB 1614216108 to previous | to last
50 21-Aug-2013 14:18 0.366 kB 1614216108 to previous | to last Hiseq 2000测序 ==> 平台项目编写模板
49 21-Aug-2013 10:38 0.357 kB qinnan to previous | to last 平台项目编写模板 ==> Hiseq 2000测序
48 18-Aug-2013 19:55 0.353 kB 人工肝 to previous | to last
47 18-Aug-2013 19:55 0.354 kB 人工肝 to previous | to last
46 18-Aug-2013 19:49 0.326 kB 人工肝 to previous | to last
45 18-Aug-2013 19:46 0.312 kB 人工肝 to previous | to last
44 18-Aug-2013 19:42 0.284 kB 人工肝 to previous | to last
43 18-Aug-2013 19:38 0.27 kB 人工肝 to previous | to last
42 18-Aug-2013 19:37 0.257 kB 人工肝 to previous | to last
41 18-Aug-2013 19:36 0.255 kB 人工肝 to previous | to last
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